After two years, the Galaxy Genome Annotation team has finally finished their much awaited tutorial on doing Genome Annotation in Galaxy! Genome annotation is a time consuming and largely manual process as it requires the synthesis of a significant amount of varied information to make inferences, a process tha cannot be accomplished without an interactive editor. To support this, Apollo was deployed on UseGalaxy.eu. Over the past two years the GGA project has pushed forward apollo and tooling around it, and it finally culminates in this look at how to annotated your genomes in Apollo.
Full StoryAnthony Bretaudeau
Affiliations
Contributions
The following list includes only slides and tutorials where the individual or organisation has been added to the contributor list. This may not include the sum total of their contributions to the training materials (e.g. GTN css or design, tutorial datasets, workflow development, etc.) unless described by a news post.
Editorial Roles
This contributor has taken on additional responsibilities as an editor for the following topics. They are responsible for ensuring that the content is up to date, accurate, and follows GTN best practices.
- Genome Annotation
- GMOD
- Genome annotation for eukaryotes
- Genome annotation for prokaryotes
- Gallantries Grant - Intellectual Output 3 - Data stewardship, federation, standardisation, and collaboration
Tutorials
- Using Galaxy and Managing your Data / InterMine integration with Galaxy π§
- Using Galaxy and Managing your Data / RStudio in Galaxy π§
- Galaxy Server administration / Deploying a compute cluster in OpenStack via Terraform π§
- Galaxy Server administration / Galaxy Installation with Ansible π
- Galaxy Server administration / Galaxy Interactive Tools βοΈ
- Galaxy Server administration / Use Apptainer containers for running Galaxy jobs π§
- Galaxy Server administration / Data Libraries π§
- Galaxy Server administration / Ansible π§
- Galaxy Server administration / Performant Uploads with TUS π§
- Galaxy Server administration / Distributed Object Storage π§
- Galaxy Server administration / Automation with Jenkins π§
- Galaxy Server administration / Galaxy Tool Management with Ephemeris π§
- Galaxy Server administration / Galaxy Monitoring with Reports π§
- Visualisation / Genomic Data Visualisation with JBrowse π§
- Visualisation / Visualisation with Circos π§
- Single Cell / Downstream Single-cell RNA analysis with RaceID π§
- Single Cell / Pre-processing of Single-Cell RNA Data π§
- Single Cell / Single-cell quality control with scater π§
- Single Cell / Clustering 3K PBMCs with Scanpy π§
- Computational chemistry / High Throughput Molecular Dynamics and Analysis π§
- Teaching and Hosting Galaxy training / Course Builder π§
- Teaching and Hosting Galaxy training / Galaxy Admin Training π§
- Teaching and Hosting Galaxy training / Running a workshop as instructor π§
- Teaching and Hosting Galaxy training / Asynchronous training π§
- Ecology / Species distribution modeling π§
- Assembly / Genome assembly using PacBio data βοΈ π§
- Assembly / Making sense of a newly assembled genome π§
- Assembly / Chloroplast genome assembly π§
- Assembly / Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data π§
- Assembly / Genome Assembly Quality Control βοΈ π§
- Assembly / Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data) π§
- Assembly / Unicycler Assembly π§
- Sequence analysis / Mapping π§
- Sequence analysis / Quality Control βοΈ π§
- Sequence analysis / SARS-CoV-2 Viral Sample Alignment and Variant Visualization π§
- Statistics and machine learning / Introduction to Machine Learning using R π§
- Proteomics / Proteogenomics 1: Database Creation π§
- Contributing to the Galaxy Training Material / Including a new topic π§
- Contributing to the Galaxy Training Material / Creating content in Markdown π§
- Contributing to the Galaxy Training Material / Creating a new tutorial π§
- Foundations of Data Science / Advanced CLI in Galaxy π§
- Foundations of Data Science / Advanced R in Galaxy π§
- Foundations of Data Science / SQL with R π§
- Foundations of Data Science / Advanced SQL π§
- Foundations of Data Science / Make & Snakemake π§
- Foundations of Data Science / Introduction to Python π§
- Foundations of Data Science / dplyr & tidyverse for data processing π§
- Foundations of Data Science / SQL Educational Game - Murder Mystery π§
- Foundations of Data Science / Advanced Python π§
- Foundations of Data Science / R basics in Galaxy π§
- Foundations of Data Science / Variant Calling Workflow π§
- Foundations of Data Science / CLI basics π§
- Foundations of Data Science / CLI Educational Game - Bashcrawl π§
- Foundations of Data Science / Plotting in Python π§
- Foundations of Data Science / Introduction to SQL π§
- Foundations of Data Science / SQL with Python π§
- Transcriptomics / De novo transcriptome reconstruction with RNA-Seq π§
- Transcriptomics / De novo transcriptome assembly, annotation, and differential expression analysis βοΈ
- Transcriptomics / RNA-Seq analysis with AskOmics Interactive Tool βοΈ π§
- Transcriptomics / RNA Seq Counts to Viz in R π§
- Transcriptomics / RNA-seq Alignment with STAR π§
- Variant Analysis / Calling very rare variants π§
- Variant Analysis / M. tuberculosis Variant Analysis π§
- Variant Analysis / Microbial Variant Calling π§
- Genome Annotation / Long non-coding RNAs (lncRNAs) annotation with FEELnc π π§
- Genome Annotation / Functional annotation of protein sequences βοΈ
- Genome Annotation / Refining Genome Annotations with Apollo (prokaryotes) βοΈ π§
- Genome Annotation / Comparative gene analysis in unannotated genomes π§
- Genome Annotation / Masking repeats with RepeatMasker βοΈ π§
- Genome Annotation / Identification of AMR genes in an assembled bacterial genome π§
- Genome Annotation / Genome annotation with Funannotate βοΈ π§
- Genome Annotation / Genome annotation with Maker βοΈ π§
- Genome Annotation / Genome annotation with Helixer βοΈ π§
- Genome Annotation / Genome annotation with Maker (short) βοΈ π§
- Genome Annotation / Genome annotation with Prokka π§
- Genome Annotation / CRISPR screen analysis π§
- Genome Annotation / Creating an Official Gene Set βοΈ π§
- Genome Annotation / Bacterial Genome Annotation π§
- Genome Annotation / Refining Genome Annotations with Apollo (eukaryotes) βοΈ π§
- Development in Galaxy / Galaxy Interactive Tools βοΈ π§
- Development in Galaxy / Creating Galaxy tools from Conda Through Deployment π§
- Development in Galaxy / Contributing to BioBlend as a developer π§
- Introduction to Galaxy Analyses / Galaxy Basics for everyone π§
Slides
- Galaxy Server administration / Galaxy Interactive Tools βοΈ π§
- Galaxy Server administration / Storage Management π§
- Galaxy Server administration / Ansible π§
- Galaxy Server administration / Server: Other π§
- Visualisation / JBrowse π§
- Visualisation / Visualisations in Galaxy π§
- Visualisation / Circos π§
- Assembly / An introduction to get started in genome assembly and annotation π π§
- Assembly / Genome assembly quality control. βοΈ π§
- Assembly / Deeper look into Genome Assembly algorithms βοΈ
- Sequence analysis / Mapping π§
- Sequence analysis / Quality Control βοΈ π§
- Transcriptomics / Integrate and query local datasets and distant RDF data with AskOmics using Semantic Web technologies βοΈ π§
- Genome Annotation / Refining Genome Annotations with Apollo βοΈ π§
- Genome Annotation / Introduction to Genome Annotation βοΈ π§
- Development in Galaxy / Tool Shed: sharing Galaxy tools βοΈ π§
- Development in Galaxy / Tool Dependencies and Conda π§
- Development in Galaxy / Tool development and integration into Galaxy βοΈ π§
- Development in Galaxy / Prerequisites for building software/conda packages βοΈ π§
- Development in Galaxy / Galaxy Code Architecture π§
- Introduction to Galaxy Analyses / Options for using Galaxy βοΈ
- Introduction to Galaxy Analyses / Introduction to Galaxy π§
Video Recordings
- Genome Annotation / Refining Genome Annotations with Apollo π¬
- Genome Annotation / Genome annotation with Prokka π¬
- Galaxy Server administration / Galaxy Interactive Tools π¬ π£
- Genome Annotation / Long non-coding RNAs (lncRNAs) annotation with FEELnc π¬
- Genome Annotation / Refining Genome Annotations with Apollo (prokaryotes) π¬ π£
- Genome Annotation / Masking repeats with RepeatMasker π¬ π£
- Genome Annotation / Genome annotation with Funannotate π¬ π£
- Genome Annotation / Genome annotation with Prokka π¬ π£
Events
- Galaxy Training Academy 2024 π§βπ«
GitHub Activity
github Issues Reported
64 Merged Pull Requests
See all of the github Pull Requests and github Commits by Anthony Bretaudeau.
-
Add eurosciencegateway affiliation to some GGA stuff
template-and-toolsgenome-annotation
-
Annotation learning paths
template-and-toolsgenome-annotation
-
FAIR intro: fix typos
template-and-tools
-
Apollo slides: fix overlapping text
genome-annotation
-
Repeatmasking and funannotate: pre-smorg review
genome-annotationfaqsre-record
Reviewed 62 PRs
We love our community reviewing each other's work!
-
Add jupyterlab stuff & other small updates
dev
-
Add Helixer training
genome-annotation
-
Update slides.html
genome-annotation
-
Add new informations: Helixer, compleasm and OMArk
genome-annotation
-
Adding EDAM topics to genome annotation gene centric tutorial
genome-annotation
News
As part of our work under the Gallantries Grant we are establishing a new topic within the GTN specifically focused on Data Science skills. This topic will include tutorials covering things such as:
Full StoryWe have just finished a new collection of training material for genome assembly and annotation! You now have access to slides and tutorials, focused on the complete analysis of the genome of a fungus species (Mucor mucedo).
Full StoryAuthor(s) | Helena Rasche Anthony Bretaudeau |
Infrastructure | Helena Rasche |
Building upon the work previously done for the SARS-Cov-2 Topic we have further expanded the βtag based topicsβ to support a new GMOD topic.
Full StoryExternal Links
Favourite Topics
Favourite Formats