Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoHelena Rasche avatar Helena Rascheorcid logoMateo Boudet avatar Mateo BoudetNathan Dunn avatar Nathan DunnNiall Beard avatar Niall Beardorcid logoSaskia Hiltemann avatar Saskia HiltemannEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoDeepti Varshney avatar Deepti VarshneyHélène Chiapello avatar Hélène Chiapelloorcid logoTristan Reynolds avatar Tristan ReynoldsTorsten Seemann avatar Torsten Seemannorcid logoMaria Doyle avatar Maria Doyleorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaim Momin avatar Saim MominAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMiaomiao Zhou avatar Miaomiao ZhouValentin Loux avatar Valentin Louxorcid logoBérénice Batut avatar Bérénice Batutorcid logoPaul Zierep avatar Paul Ziereporcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnika Erxleben avatar Anika ErxlebenJasper Ouwerkerk avatar Jasper OuwerkerkEkaterina Polkh avatar Ekaterina PolkhGuillaume Gautreau avatar Guillaume GautreauWilliam Durand avatar William Durandorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoLaura Leroi avatar Laura Leroiorcid logoBjörn Grüning avatar Björn GrüningKenji Fujihara avatar Kenji Fujiharaorcid logoTeresa Müller avatar Teresa MüllerBazante Sanders avatar Bazante Sandersorcid logoFelicitas Kindel avatar Felicitas KindelGildas Le Corguillé avatar Gildas Le Corguilléorcid logoCristóbal Gallardo avatar Cristóbal GallardoTwishi Gulati avatar Twishi Gulatiorcid logoErwan Corre avatar Erwan Correorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnna Syme avatar Anna SymeSimon Bray avatar Simon Bray

Funding

These individuals or organisations provided funding support for the development of this resource