Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSaim Momin avatar Saim MominAnton Nekrutenko avatar Anton Nekrutenkoorcid logoBérénice Batut avatar Bérénice Batutorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti VarshneyValentin Loux avatar Valentin Louxorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoMaria Doyle avatar Maria Doyleorcid logoCristóbal Gallardo avatar Cristóbal GallardoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoHelena Rasche avatar Helena Rascheorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoFelicitas Kindel avatar Felicitas KindelTorsten Seemann avatar Torsten SeemannSimon Bray avatar Simon Brayorcid logoRomane LIBOUBAN avatar Romane LIBOUBANTwishi Gulati avatar Twishi GulatiNiall Beard avatar Niall BeardKenji Fujihara avatar Kenji Fujiharaorcid logoJonathan Kreplak avatar Jonathan KreplakNathan Dunn avatar Nathan DunnHélène Chiapello avatar Hélène Chiapelloorcid logoLaura Leroi avatar Laura Leroiorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnna Syme avatar Anna Symeorcid logoNiklas Mayle avatar Niklas Mayleorcid logoBjörn Grüning avatar Björn Grüningorcid logoAlexandre Cormier avatar Alexandre CormierJasper Ouwerkerk avatar Jasper OuwerkerkWilliam Durand avatar William Durandorcid logoErwan Corre avatar Erwan Correorcid logoSimon Gladman avatar Simon Gladmanorcid logoTeresa Müller avatar Teresa MüllerGuillaume Gautreau avatar Guillaume GautreauBazante Sanders avatar Bazante SandersGildas Le Corguillé avatar Gildas Le Corguilléorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoPaul Zierep avatar Paul ZierepEkaterina Polkh avatar Ekaterina Polkhorcid logoNicola Soranzo avatar Nicola Soranzo

Funding

These individuals or organisations provided funding support for the development of this resource