Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Twishi Gulati avatar Twishi Gulatiorcid logoStéphanie Robin avatar Stéphanie RobinTorsten Seemann avatar Torsten SeemannGuillaume Gautreau avatar Guillaume GautreauEkaterina Polkh avatar Ekaterina Polkhorcid logoJonathan Kreplak avatar Jonathan KreplakHélène Chiapello avatar Hélène ChiapelloJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoTristan Reynolds avatar Tristan ReynoldsNiall Beard avatar Niall Beardorcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoRomane LIBOUBAN avatar Romane LIBOUBANSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSaim Momin avatar Saim Mominorcid logoPaul Zierep avatar Paul ZierepNathan Dunn avatar Nathan Dunnorcid logoMaria Doyle avatar Maria DoyleKenji Fujihara avatar Kenji Fujiharaorcid logoMateo Boudet avatar Mateo Boudetorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoErwan Corre avatar Erwan CorreBazante Sanders avatar Bazante Sandersorcid logoBjörn Grüning avatar Björn Grüningorcid logoAnika Erxleben avatar Anika ErxlebenValentin Loux avatar Valentin Louxorcid logoNiklas Mayle avatar Niklas Mayleorcid logoSimon Gladman avatar Simon GladmanWilliam Durand avatar William Durandorcid logoBérénice Batut avatar Bérénice Batutorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTeresa Müller avatar Teresa Müllerorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSaskia Hiltemann avatar Saskia HiltemannGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnna Syme avatar Anna SymeAnton Nekrutenko avatar Anton Nekrutenkoorcid logoLaura Leroi avatar Laura LeroiEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDeepti Varshney avatar Deepti Varshney

Funding

These individuals or organisations provided funding support for the development of this resource