Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMiaomiao Zhou avatar Miaomiao ZhouWilliam Durand avatar William Durandorcid logoBérénice Batut avatar Bérénice Batutorcid logoSaskia Hiltemann avatar Saskia HiltemannNathan Dunn avatar Nathan Dunnorcid logoMateo Boudet avatar Mateo Boudetorcid logoBjörn Grüning avatar Björn Grüningorcid logoCristóbal Gallardo avatar Cristóbal GallardoEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilKenji Fujihara avatar Kenji FujiharaGildas Le Corguillé avatar Gildas Le Corguilléorcid logoLaura Leroi avatar Laura Leroiorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoPaul Zierep avatar Paul Ziereporcid logoErwan Corre avatar Erwan CorreGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall Beardorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnna Syme avatar Anna SymeBazante Sanders avatar Bazante Sandersorcid logoHelena Rasche avatar Helena RascheTwishi Gulati avatar Twishi GulatiEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoFelicitas Kindel avatar Felicitas KindelSimon Bray avatar Simon BrayTorsten Seemann avatar Torsten SeemannJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMaria Doyle avatar Maria DoyleValentin Loux avatar Valentin Louxorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola SoranzoHélène Chiapello avatar Hélène Chiapelloorcid logoTeresa Müller avatar Teresa Müllerorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoDeepti Varshney avatar Deepti VarshneyAnton Nekrutenko avatar Anton Nekrutenko

Funding

These individuals or organisations provided funding support for the development of this resource