Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoHelena Rasche avatar Helena Rascheorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoBérénice Batut avatar Bérénice Batutorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoNiklas Mayle avatar Niklas MayleGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall Beardorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnika Erxleben avatar Anika ErxlebenTwishi Gulati avatar Twishi Gulatiorcid logoBjörn Grüning avatar Björn GrüningHélène Chiapello avatar Hélène ChiapelloJasper Ouwerkerk avatar Jasper OuwerkerkNathan Dunn avatar Nathan DunnSimon Bray avatar Simon Brayorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura Leroiorcid logoSaim Momin avatar Saim Mominorcid logoAnna Syme avatar Anna Symeorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoCristóbal Gallardo avatar Cristóbal GallardoBazante Sanders avatar Bazante SandersGildas Le Corguillé avatar Gildas Le Corguilléorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTristan Reynolds avatar Tristan ReynoldsValentin Loux avatar Valentin Louxorcid logoSimon Gladman avatar Simon Gladmanorcid logoDelphine Lariviere avatar Delphine LariviereAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaskia Hiltemann avatar Saskia HiltemannEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMaria Doyle avatar Maria Doyleorcid logoRomane LIBOUBAN avatar Romane LIBOUBANEkaterina Polkh avatar Ekaterina PolkhTorsten Seemann avatar Torsten Seemannorcid logoErwan Corre avatar Erwan CorreKenji Fujihara avatar Kenji FujiharaWilliam Durand avatar William Durandorcid logoTeresa Müller avatar Teresa Müllerorcid logoMiaomiao Zhou avatar Miaomiao Zhou

Funding

These individuals or organisations provided funding support for the development of this resource