Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Simon Bray avatar Simon Brayorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMateo Boudet avatar Mateo BoudetGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall BeardJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnna Syme avatar Anna Symeorcid logoTristan Reynolds avatar Tristan ReynoldsTwishi Gulati avatar Twishi GulatiTorsten Seemann avatar Torsten Seemannorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoSaim Momin avatar Saim MominGildas Le Corguillé avatar Gildas Le Corguilléorcid logoPaul Zierep avatar Paul Ziereporcid logoNicola Soranzo avatar Nicola Soranzoorcid logoBjörn Grüning avatar Björn Grüningorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan KreplakEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoLaura Leroi avatar Laura Leroiorcid logoFelicitas Kindel avatar Felicitas KindelAnton Nekrutenko avatar Anton Nekrutenkoorcid logoTeresa Müller avatar Teresa MüllerKenji Fujihara avatar Kenji FujiharaEkaterina Polkh avatar Ekaterina Polkhorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMaria Doyle avatar Maria Doyleorcid logoSimon Gladman avatar Simon GladmanValentin Loux avatar Valentin Louxorcid logoHelena Rasche avatar Helena Rascheorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAlexandre Cormier avatar Alexandre CormierHélène Chiapello avatar Hélène ChiapelloBazante Sanders avatar Bazante SandersNathan Dunn avatar Nathan DunnWilliam Durand avatar William Durandorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBérénice Batut avatar Bérénice Batutorcid logoErwan Corre avatar Erwan Corre

Funding

These individuals or organisations provided funding support for the development of this resource