Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Kenji Fujihara avatar Kenji Fujiharaorcid logoPaul Zierep avatar Paul ZierepSimon Bray avatar Simon BrayValentin Loux avatar Valentin LouxWilliam Durand avatar William DurandAnton Nekrutenko avatar Anton NekrutenkoNiall Beard avatar Niall Beardorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoHelena Rasche avatar Helena Rascheorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoLaura Leroi avatar Laura LeroiGuillaume Gautreau avatar Guillaume Gautreauorcid logoJonathan Kreplak avatar Jonathan KreplakTwishi Gulati avatar Twishi Gulatiorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBérénice Batut avatar Bérénice Batutorcid logoMateo Boudet avatar Mateo BoudetEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAnna Syme avatar Anna Symeorcid logoRomane LIBOUBAN avatar Romane LIBOUBANTorsten Seemann avatar Torsten Seemannorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoBjörn Grüning avatar Björn GrüningEkaterina Polkh avatar Ekaterina PolkhNathan Dunn avatar Nathan Dunnorcid logoErwan Corre avatar Erwan CorreJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola SoranzoGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoMaria Doyle avatar Maria DoyleHélène Chiapello avatar Hélène ChiapelloBazante Sanders avatar Bazante Sanders

Funding

These individuals or organisations provided funding support for the development of this resource