Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnna Syme avatar Anna SymeGuillaume Gautreau avatar Guillaume GautreauJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoCristóbal Gallardo avatar Cristóbal GallardoBazante Sanders avatar Bazante Sandersorcid logoRomane LIBOUBAN avatar Romane LIBOUBANSimon Bray avatar Simon Brayorcid logoBjörn Grüning avatar Björn Grüningorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoPaul Zierep avatar Paul Ziereporcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoLaura Leroi avatar Laura Leroiorcid logoSaim Momin avatar Saim MominEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilNathan Dunn avatar Nathan Dunnorcid logoTeresa Müller avatar Teresa Müllerorcid logoNicola Soranzo avatar Nicola SoranzoEkaterina Polkh avatar Ekaterina Polkhorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBérénice Batut avatar Bérénice BatutHélène Chiapello avatar Hélène ChiapelloWilliam Durand avatar William Durandorcid logoNiklas Mayle avatar Niklas MayleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoJonathan Kreplak avatar Jonathan KreplakNiall Beard avatar Niall Beardorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoHelena Rasche avatar Helena RascheAnton Nekrutenko avatar Anton NekrutenkoValentin Loux avatar Valentin Louxorcid logoMaria Doyle avatar Maria Doyleorcid logoErwan Corre avatar Erwan CorreTorsten Seemann avatar Torsten SeemannTwishi Gulati avatar Twishi GulatiKenji Fujihara avatar Kenji Fujiharaorcid logoStéphanie Robin avatar Stéphanie Robin

Funding

These individuals or organisations provided funding support for the development of this resource