Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Pangenome analysis with Roary
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

William Durand avatar William Durandorcid logoSaskia Hiltemann avatar Saskia HiltemannNathan Dunn avatar Nathan Dunnorcid logoNicola Soranzo avatar Nicola SoranzoEkaterina Polkh avatar Ekaterina Polkhorcid logoHelena Rasche avatar Helena RascheEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTwishi Gulati avatar Twishi Gulatiorcid logoSimon Gladman avatar Simon Gladmanorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoPaul Zierep avatar Paul Ziereporcid logoBérénice Batut avatar Bérénice Batutorcid logoTeresa Müller avatar Teresa MüllerKenji Fujihara avatar Kenji Fujiharaorcid logoMaria Doyle avatar Maria Doyleorcid logoFelicitas Kindel avatar Felicitas KindelHélène Chiapello avatar Hélène Chiapelloorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoNiklas Mayle avatar Niklas MayleNiall Beard avatar Niall Beardorcid logoAlexandre Cormier avatar Alexandre CormierGildas Le Corguillé avatar Gildas Le CorguilléTorsten Seemann avatar Torsten Seemannorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoSaim Momin avatar Saim Mominorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBjörn Grüning avatar Björn GrüningBazante Sanders avatar Bazante Sandersorcid logoMiaomiao Zhou avatar Miaomiao ZhouGuillaume Gautreau avatar Guillaume GautreauValentin Loux avatar Valentin Louxorcid logoMateo Boudet avatar Mateo Boudetorcid logoTristan Reynolds avatar Tristan ReynoldsJasper Ouwerkerk avatar Jasper OuwerkerkSimon Bray avatar Simon Brayorcid logoAnna Syme avatar Anna Symeorcid logoLaura Leroi avatar Laura LeroiAnton Nekrutenko avatar Anton Nekrutenkoorcid logoErwan Corre avatar Erwan Correorcid logoAnika Erxleben avatar Anika Erxleben

Funding

These individuals or organisations provided funding support for the development of this resource