Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

William Durand avatar William Durandorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoRomane LIBOUBAN avatar Romane LIBOUBANTorsten Seemann avatar Torsten Seemannorcid logoHelena Rasche avatar Helena Rascheorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSaim Momin avatar Saim Mominorcid logoAnna Syme avatar Anna Symeorcid logoPaul Zierep avatar Paul ZierepAnton Nekrutenko avatar Anton Nekrutenkoorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoMateo Boudet avatar Mateo Boudetorcid logoMiaomiao Zhou avatar Miaomiao ZhouKenji Fujihara avatar Kenji FujiharaGildas Le Corguillé avatar Gildas Le Corguilléorcid logoSolenne Correard avatar Solenne CorreardNathan Dunn avatar Nathan DunnTwishi Gulati avatar Twishi GulatiJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDelphine Lariviere avatar Delphine LariviereGuillaume Gautreau avatar Guillaume GautreauDonny Vrins avatar Donny VrinsEkaterina Polkh avatar Ekaterina Polkhorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTeresa Müller avatar Teresa Müllerorcid logoNicola Soranzo avatar Nicola SoranzoValentin Loux avatar Valentin LouxBazante Sanders avatar Bazante Sandersorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoBjörn Grüning avatar Björn Grüningorcid logoAnika Erxleben avatar Anika ErxlebenEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia HiltemannNiall Beard avatar Niall Beardorcid logoMaria Doyle avatar Maria Doyleorcid logoErwan Corre avatar Erwan Correorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoLaura Leroi avatar Laura LeroiHélène Chiapello avatar Hélène ChiapelloSimon Bray avatar Simon Brayorcid logoVesselin Baev avatar Vesselin Baevorcid logoBérénice Batut avatar Bérénice Batutorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource