Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoNicola Soranzo avatar Nicola SoranzoTorsten Seemann avatar Torsten SeemannTwishi Gulati avatar Twishi Gulatiorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoPaul Zierep avatar Paul Ziereporcid logoBérénice Batut avatar Bérénice BatutJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoRomane LIBOUBAN avatar Romane LIBOUBANBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin Louxorcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenKenji Fujihara avatar Kenji Fujiharaorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoNiklas Mayle avatar Niklas Mayleorcid logoDelphine Lariviere avatar Delphine LariviereGildas Le Corguillé avatar Gildas Le CorguilléGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa MüllerEkaterina Polkh avatar Ekaterina Polkhorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoFelicitas Kindel avatar Felicitas KindelWilliam Durand avatar William Durandorcid logoLaura Leroi avatar Laura LeroiEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMaria Doyle avatar Maria DoyleNathan Dunn avatar Nathan Dunnorcid logoSimon Gladman avatar Simon Gladmanorcid logoMateo Boudet avatar Mateo Boudetorcid logoSaim Momin avatar Saim Mominorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnna Syme avatar Anna SymeSimon Bray avatar Simon Brayorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAlexandre Cormier avatar Alexandre CormierAnton Nekrutenko avatar Anton NekrutenkoHélène Chiapello avatar Hélène ChiapelloNiall Beard avatar Niall Beardorcid logoErwan Corre avatar Erwan Correorcid logoBjörn Grüning avatar Björn Grüningorcid logoCristóbal Gallardo avatar Cristóbal Gallardo

Funding

These individuals or organisations provided funding support for the development of this resource