Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnna Syme avatar Anna SymeAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAnika Erxleben avatar Anika ErxlebenTwishi Gulati avatar Twishi GulatiTorsten Seemann avatar Torsten SeemannJasper Ouwerkerk avatar Jasper OuwerkerkEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoBjörn Grüning avatar Björn Grüningorcid logoMaria Doyle avatar Maria Doyleorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaim Momin avatar Saim MominEkaterina Polkh avatar Ekaterina Polkhorcid logoBérénice Batut avatar Bérénice Batutorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoSimon Gladman avatar Simon GladmanBazante Sanders avatar Bazante Sandersorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoLaura Leroi avatar Laura LeroiHélène Chiapello avatar Hélène ChiapelloValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNiall Beard avatar Niall Beardorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAlexandre Cormier avatar Alexandre CormierKenji Fujihara avatar Kenji Fujiharaorcid logoDelphine Lariviere avatar Delphine LariviereWilliam Durand avatar William Durandorcid logoErwan Corre avatar Erwan Correorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTristan Reynolds avatar Tristan ReynoldsGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa Müllerorcid logoMateo Boudet avatar Mateo BoudetGildas Le Corguillé avatar Gildas Le Corguilléorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoHelena Rasche avatar Helena RascheSimon Bray avatar Simon Brayorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoPaul Zierep avatar Paul ZierepNathan Dunn avatar Nathan Dunn

Funding

These individuals or organisations provided funding support for the development of this resource