Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Valentin Loux avatar Valentin Louxorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSimon Gladman avatar Simon GladmanEkaterina Polkh avatar Ekaterina Polkhorcid logoLaura Leroi avatar Laura LeroiTorsten Seemann avatar Torsten Seemannorcid logoHelena Rasche avatar Helena RascheWilliam Durand avatar William DurandGuillaume Gautreau avatar Guillaume Gautreauorcid logoSaskia Hiltemann avatar Saskia HiltemannEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilSimon Bray avatar Simon BrayNathan Dunn avatar Nathan Dunnorcid logoFelicitas Kindel avatar Felicitas KindelJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoTeresa Müller avatar Teresa Müllerorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoTristan Reynolds avatar Tristan ReynoldsTwishi Gulati avatar Twishi GulatiGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria Doyleorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMateo Boudet avatar Mateo Boudetorcid logoMiaomiao Zhou avatar Miaomiao ZhouHélène Chiapello avatar Hélène ChiapelloNiall Beard avatar Niall Beardorcid logoAnna Syme avatar Anna SymeAnton Nekrutenko avatar Anton Nekrutenkoorcid logoPaul Zierep avatar Paul Ziereporcid logoDelphine Lariviere avatar Delphine LariviereBazante Sanders avatar Bazante Sandersorcid logoBjörn Grüning avatar Björn Grüningorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoSaim Momin avatar Saim MominKenji Fujihara avatar Kenji Fujiharaorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoErwan Corre avatar Erwan Correorcid logoBérénice Batut avatar Bérénice Batut

Funding

These individuals or organisations provided funding support for the development of this resource