Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoNicola Soranzo avatar Nicola Soranzoorcid logoErwan Corre avatar Erwan Correorcid logoAnika Erxleben avatar Anika ErxlebenWilliam Durand avatar William DurandEkaterina Polkh avatar Ekaterina PolkhJasper Ouwerkerk avatar Jasper OuwerkerkSimon Bray avatar Simon BrayKenji Fujihara avatar Kenji FujiharaTorsten Seemann avatar Torsten SeemannNathan Dunn avatar Nathan DunnEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoLaura Leroi avatar Laura LeroiNiall Beard avatar Niall BeardBazante Sanders avatar Bazante Sandersorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnna Syme avatar Anna SymeGuillaume Gautreau avatar Guillaume Gautreauorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoPaul Zierep avatar Paul Ziereporcid logoTeresa Müller avatar Teresa Müllerorcid logoCristóbal Gallardo avatar Cristóbal GallardoTwishi Gulati avatar Twishi Gulatiorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoHelena Rasche avatar Helena Rascheorcid logoDeepti Varshney avatar Deepti VarshneyAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMateo Boudet avatar Mateo Boudetorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSimon Gladman avatar Simon Gladmanorcid logoBjörn Grüning avatar Björn Grüningorcid logoFelicitas Kindel avatar Felicitas KindelHélène Chiapello avatar Hélène ChiapelloValentin Loux avatar Valentin LouxGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria Doyleorcid logoBérénice Batut avatar Bérénice Batutorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource