Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Torsten Seemann avatar Torsten SeemannNathan Dunn avatar Nathan Dunnorcid logoSaim Momin avatar Saim Mominorcid logoErwan Corre avatar Erwan CorreGuillaume Gautreau avatar Guillaume Gautreauorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoPaul Zierep avatar Paul ZierepHélène Chiapello avatar Hélène ChiapelloEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBANBazante Sanders avatar Bazante SandersWilliam Durand avatar William DurandSimon Bray avatar Simon Brayorcid logoBjörn Grüning avatar Björn GrüningValentin Loux avatar Valentin Louxorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMaria Doyle avatar Maria Doyleorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoNicola Soranzo avatar Nicola SoranzoAnton Nekrutenko avatar Anton NekrutenkoKenji Fujihara avatar Kenji Fujiharaorcid logoTeresa Müller avatar Teresa MüllerJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBérénice Batut avatar Bérénice BatutEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoAnna Syme avatar Anna Symeorcid logoHelena Rasche avatar Helena Rascheorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoLaura Leroi avatar Laura Leroiorcid logoMateo Boudet avatar Mateo Boudetorcid logoSimon Gladman avatar Simon GladmanNiall Beard avatar Niall BeardTwishi Gulati avatar Twishi Gulatiorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan Kreplak

Funding

These individuals or organisations provided funding support for the development of this resource