Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Jasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDelphine Lariviere avatar Delphine LariviereNiall Beard avatar Niall BeardGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan KreplakAnton Nekrutenko avatar Anton Nekrutenkoorcid logoBérénice Batut avatar Bérénice BatutGuillaume Gautreau avatar Guillaume Gautreauorcid logoTristan Reynolds avatar Tristan ReynoldsHélène Chiapello avatar Hélène Chiapelloorcid logoPaul Zierep avatar Paul Ziereporcid logoBjörn Grüning avatar Björn GrüningEkaterina Polkh avatar Ekaterina PolkhSimon Bray avatar Simon Brayorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMaria Doyle avatar Maria DoyleNathan Dunn avatar Nathan Dunnorcid logoNicola Soranzo avatar Nicola SoranzoTorsten Seemann avatar Torsten Seemannorcid logoAnna Syme avatar Anna Symeorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoErwan Corre avatar Erwan Correorcid logoSaim Momin avatar Saim Mominorcid logoLaura Leroi avatar Laura LeroiTwishi Gulati avatar Twishi Gulatiorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti VarshneyKenji Fujihara avatar Kenji Fujiharaorcid logoHelena Rasche avatar Helena RascheBazante Sanders avatar Bazante Sandersorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoNiklas Mayle avatar Niklas Mayleorcid logoCristóbal Gallardo avatar Cristóbal GallardoWilliam Durand avatar William DurandEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnthony Bretaudeau avatar Anthony BretaudeauValentin Loux avatar Valentin Loux

Funding

These individuals or organisations provided funding support for the development of this resource