Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Jasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria DoyleTorsten Seemann avatar Torsten Seemannorcid logoSaskia Hiltemann avatar Saskia HiltemannAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoNicola Soranzo avatar Nicola SoranzoDeepti Varshney avatar Deepti Varshneyorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoLaura Leroi avatar Laura Leroiorcid logoMateo Boudet avatar Mateo BoudetEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilSimon Bray avatar Simon Brayorcid logoAnika Erxleben avatar Anika ErxlebenNiall Beard avatar Niall Beardorcid logoHelena Rasche avatar Helena Rascheorcid logoErwan Corre avatar Erwan CorreTeresa Müller avatar Teresa Müllerorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBérénice Batut avatar Bérénice BatutBazante Sanders avatar Bazante SandersEkaterina Polkh avatar Ekaterina PolkhNathan Dunn avatar Nathan Dunnorcid logoPaul Zierep avatar Paul ZierepTwishi Gulati avatar Twishi GulatiWilliam Durand avatar William Durandorcid logoAnna Syme avatar Anna Symeorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSimon Gladman avatar Simon Gladmanorcid logoStéphanie Robin avatar Stéphanie RobinKenji Fujihara avatar Kenji Fujihara

Funding

These individuals or organisations provided funding support for the development of this resource