Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSimon Gladman avatar Simon GladmanTorsten Seemann avatar Torsten SeemannBazante Sanders avatar Bazante Sandersorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoPaul Zierep avatar Paul Ziereporcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoLaura Leroi avatar Laura Leroiorcid logoBjörn Grüning avatar Björn Grüningorcid logoMaria Doyle avatar Maria DoyleEkaterina Polkh avatar Ekaterina Polkhorcid logoTeresa Müller avatar Teresa Müllerorcid logoJonathan Kreplak avatar Jonathan KreplakJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBérénice Batut avatar Bérénice Batutorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnika Erxleben avatar Anika ErxlebenHélène Chiapello avatar Hélène Chiapelloorcid logoErwan Corre avatar Erwan CorreEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoNicola Soranzo avatar Nicola SoranzoNiall Beard avatar Niall Beardorcid logoMateo Boudet avatar Mateo Boudetorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnna Syme avatar Anna SymeKenji Fujihara avatar Kenji FujiharaValentin Loux avatar Valentin LouxTwishi Gulati avatar Twishi Gulatiorcid logoHelena Rasche avatar Helena RascheGildas Le Corguillé avatar Gildas Le CorguilléWilliam Durand avatar William Durandorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoAnthony Bretaudeau avatar Anthony BretaudeauAnton Nekrutenko avatar Anton NekrutenkoGuillaume Gautreau avatar Guillaume GautreauSimon Bray avatar Simon Bray

Funding

These individuals or organisations provided funding support for the development of this resource