Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Kenji Fujihara avatar Kenji Fujiharaorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi GulatiValentin Loux avatar Valentin LouxNiall Beard avatar Niall BeardAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoStéphanie Robin avatar Stéphanie RobinGildas Le Corguillé avatar Gildas Le CorguilléEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilEkaterina Polkh avatar Ekaterina PolkhTorsten Seemann avatar Torsten Seemannorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBérénice Batut avatar Bérénice Batutorcid logoErwan Corre avatar Erwan Correorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMateo Boudet avatar Mateo BoudetHélène Chiapello avatar Hélène Chiapelloorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoNicola Soranzo avatar Nicola SoranzoBazante Sanders avatar Bazante Sandersorcid logoJonathan Kreplak avatar Jonathan KreplakWilliam Durand avatar William DurandSimon Bray avatar Simon Brayorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoRomane LIBOUBAN avatar Romane LIBOUBANNathan Dunn avatar Nathan Dunnorcid logoHelena Rasche avatar Helena Rascheorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoAnna Syme avatar Anna SymeGuillaume Gautreau avatar Guillaume Gautreauorcid logoMaria Doyle avatar Maria Doyleorcid logoLaura Leroi avatar Laura Leroiorcid logoPaul Zierep avatar Paul Ziereporcid logoAnthony Bretaudeau avatar Anthony BretaudeauJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoSimon Gladman avatar Simon Gladmanorcid logoBjörn Grüning avatar Björn Grüning

Funding

These individuals or organisations provided funding support for the development of this resource