Melanie Föll

Affiliations

Contributions

The following list includes only slides and tutorials where the individual or organisation has been added to the contributor list. This may not include the sum total of their contributions to the training materials (e.g. GTN css or design, tutorial datasets, workflow development, etc.) unless described by a news post.

Editorial Roles

This contributor has taken on additional responsibilities as an editor for the following topics. They are responsible for ensuring that the content is up to date, accurate, and follows GTN best practices.

• Metabolomics
• Proteomics

Tutorials

• Proteomics / DIA Analysis using OpenSwathWorkflow
• Proteomics / Label-free data analysis using MaxQuant
• Proteomics / Library Generation for DIA Analysis
• Proteomics / Statistical analysis of DIA data
• Proteomics / MaxQuant and MSstats for the analysis of label-free data
• Proteomics / MaxQuant and MSstats for the analysis of TMT data
• Proteomics / Mass spectrometry imaging: Loading and exploring MSI data
• Metabolomics / Mass spectrometry imaging: Finding differential analytes
• Metabolomics / Mass spectrometry imaging: Examining the spatial distribution of analytes ✍️
• Introduction to Galaxy Analyses / How to reproduce published Galaxy analyses ✍️

Slides

• Proteomics / Introduction to proteomics, protein identification, quantification and statistical modelling

FAQs

• Do I need to create collections to run MaxQuant analysis or can I use single sample inputs?
• Including custom modifications into MaxQuant in Galaxy?
• Which isobaric labeled quantification methods does MaxQuant in Galaxy support?
• What is the advantage of breaking down protein to peptides before mass spec?
• Do we need a contaminant FASTA for MQ in galaxy?
• When can you use (or cannot use) Match between runs in MaxQuant?
• Do you need to merge the databases? Because you can select multiple fasta files in MaxQuant.
• If you use a mqpar file, can you include modifications that are not in the Galaxy version? For instance, propionamide (Cys alkylation by acrylamide).
• What does it mean to normalize the LFQ intensities?
• Does MaxQuant give as output possibility the PSMs and PEPs?
• For the “quantitation method” what is the default if I just leave it as “None”? Label free?
• Additional resources to learn more about proteomic data analysis
• How many proteins can be identified and quantified in shotgun proteomics?
• MSStats: what does ‘compare groups = yes’ mean? And the comparison matrix to define the contrast between the 2 groups?
• Does MaxQuant in Galaxy support TMT, iTRAQ, etc.?

Video Recordings

• Proteomics / Introduction to proteomics, protein identification, quantification and statistical modelling 💬
• Proteomics / MaxQuant and MSstats for the analysis of label-free data 💬 🗣

Events

• Galaxy Training Academy 2024 🧑‍🏫

GitHub Activity

github Issues Reported

41 Merged Pull Requests

See all of the github Pull Requests and github Commits by Melanie Föll.

• Update maxquant and msstats tutorial
  proteomics
• Update tutorial Maxquant LFQ
  proteomics
• Updating MaxQuant & MSstats tutorials
• Update links to new ftp location in PRIDE
• New GTN how to reproduce Galaxy analysis

Reviewed 12 PRs

We love our community reviewing each other's work!

• QCxMS prediction tutorial
  template-and-tools
  metabolomics
• Fixes #4406
  proteomics
• New tutotial: Metabolomics LC-MS data processing
  review-needed
• Metabolomics - LCMS preprocessing update
  review-needed
• New GTN how to reproduce Galaxy analysis

External Links

Favourite Topics

Favourite Formats