Imaging

Image analysis using Galaxy

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.

Introduction

Start here if you are new to Image Analysis in Galaxy

Lesson Slides Hands-on Recordings Input dataset Workflows
FAIR Bioimage Metadata
Data management Metadata Bioimaging
REMBI - Recommended Metadata for Biological Images – metadata guidelines for bioimaging data
Data management Metadata Bioimaging
Introduction to Image Analysis using Galaxy
Image segmentation Image thresholding Conversion Object counting Overlay Fluorescence microscopy
Overview of the Galaxy OMERO-suite - Upload images and metadata in OMERO using Galaxy
Data management Data handling Data sharing ROI selection
Where to start with bioimage analysis in Galaxy
Microscopy Bioimaging Histology

Analyses

These tutorials will help you analyse your image data

Lesson Slides Hands-on Recordings Input dataset Workflows
Analyse HeLa fluorescence siRNA screen
Image segmentation Object feature extraction Image thresholding High-throughput screening Overlay Fluorescence microscopy
End-to-End Tissue Microarray Image Analysis with Galaxy-ME
Image segmentation Tissue imaging
Nucleoli segmentation and feature extraction using CellProfiler
Image segmentation Object feature extraction Multi-channel image
Object tracking using CellProfiler
Isolated object tracking Multi-channel image Earth observation
Quantification of electrophoresis gel bands using QuPath and Galaxy imaging tools
ROI selection Conversion Object feature extraction Multi-channel image
Quantification of single-molecule RNA fluorescence in situ hybridization (smFISH) in yeast cell lines
IDR dataset Spot detection Rasterisation Overlay
Quantitative Analysis of Histological Staining Using Color Deconvolution
Microscopy Bioimaging Histology pathology
Segmentation of Anatomical Structures in Medical 3-D Images
Image segmentation Conversion Overlay Object feature extraction Medical imaging Computed tomography 3D image Volume rendering
Tracking of mitochondria and capturing mitoflashes
Isolated object tracking Temporal analysis Time-lapse data
Use Pycytominer in Galaxy for processing high dimensional image-based readouts
Object feature extraction high-throughput data cleaning cellprofiler deepprofiler
Using BioImage.IO models for image analysis in Galaxy
Image segmentation Image annotation Deep learning
Voronoi segmentation
Tessellation Image segmentation Object counting Overlay Multi-channel image

Advanced topics

Tutorials covering advanced topics

Lesson Slides Hands-on Recordings Input dataset Workflows
Content Tracking and Verification in Galaxy Workflows with ISCC-SUM
Data management Data handling Data sharing Validation
Execute a BiaPy workflow in Galaxy
Image segmentation Image annotation Deep learning Conversion Overlay 3D image Volume rendering
Parameter tuning and optimization - Evaluating nuclei segmentation with Galaxy
Image segmentation Parameter optimisation Validation
Training Custom YOLO Models for Segmentation of Bioimages
Image segmentation Image annotation Deep learning

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
AI pipeline for annotating marine species (Project Moorev - Marine)
Marine ecosystems biodiversity AI segmentation YOLO deep learning object detection

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoLeonid Kostrykin avatar Leonid Kostrykinorcid logoBeatriz Serrano-Solano avatar Beatriz Serrano-Solanoorcid logoDiana Chiang Jurado avatar Diana Chiang Jurado

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

orcid logoMarie Josse avatar Marie JosseArif ul Maula Khan avatar Arif ul Maula KhanMaria Arrate Munoz Barrutia avatar Maria Arrate Munoz Barrutiaorcid logoThomas Wollmann avatar Thomas Wollmannorcid logoLeonid Kostrykin avatar Leonid KostrykinDaniel Franco Barranco avatar Daniel Franco BarrancoKevin Payet avatar Kevin Payetorcid logoEven Moa Myklebust avatar Even Moa Myklebustorcid logoPavankumar Videm avatar Pavankumar VidemLaura Cooper avatar Laura Cooperorcid logoKrzysztof Poterlowicz avatar Krzysztof Poterlowiczorcid logoBjörn Grüning avatar Björn Grüningorcid logoReyhaneh Tavakoli-Koopaei avatar Reyhaneh Tavakoli-Koopaeiorcid logoKhaled Jum'ah avatar Khaled Jum'ahorcid logoSaskia Hiltemann avatar Saskia HiltemannErik Serrano avatar Erik Serranoorcid logoDaniela Schneider avatar Daniela Schneiderorcid logoJulia Jakiela avatar Julia JakielaArthur Barreau avatar Arthur Barreauorcid logoAnne Fouilloux avatar Anne Fouillouxorcid logoBeatriz Serrano-Solano avatar Beatriz Serrano-Solanoorcid logoRiccardo Massei avatar Riccardo Masseiorcid logoCameron Watson avatar Cameron WatsonYi Sun avatar Yi SunEnis Afgan avatar Enis Afganorcid logoAllison Creason avatar Allison Creasonorcid logoDiana Chiang Jurado avatar Diana Chiang Juradoorcid logoPaul De Geest avatar Paul De Geestorcid logoMaarten Paul avatar Maarten Paulorcid logoHelena Rasche avatar Helena Rascheorcid logoMarius van den Beek avatar Marius van den Beekorcid logoKatarzyna Kamieniecka avatar Katarzyna Kamienieckaorcid logoNadine Le Bris avatar Nadine Le Brisorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMartin Čech avatar Martin Čechorcid logoYvan Le Bras avatar Yvan Le BrasJean-Karim Hériché avatar Jean-Karim HérichéDave Bunten avatar Dave Buntenorcid logoMartin Etzrodt avatar Martin Etzrodtorcid logoAnup Kumar avatar Anup Kumarorcid logoArmin Dadras avatar Armin Dadras

Funding

These individuals or organisations provided funding support for the development of this resource

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ISYEB
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MOOREV
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ELIXIR Europe
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DFG
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OSCARS
The OSCARS project has received funding from the European Commission’s Horizon Europe Research and Innovation programme under grant agreement No. 101129751
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DASH UK
This Fellowship was funded through the ELIXIR-UK DaSH project as part of the UKRI Innovation Scholars: Data Science Training in Health and Bioscience call (DaSH). (MR/V038966/1). The project aims to embed Research Data Management (RDM) know-how into UK universities and institutes by producing and delivering training in FAIR data stewardship using ELIXIR-UK knowledge and resources.
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NFDI4Bioimage
NFDI4BIOIMAGE is a nationwide community of researchers, imaging specialists and data experts who work with biological image data. We support anyone who generates, analyses or manages microscopy and bioimaging data. Our aim is to make imaging data easier to find, access, reuse and share by providing practical tools, clear guidance and hands on training. We collaborate closely with laboratories and research communities across Germany to understand their needs and develop solutions that help with real everyday challenges. Whether you are building new workflows, exploring advanced analysis methods or improving your data management, NFDI4BIOIMAGE offers support, expertise and a welcoming network.
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OSCARS-FIESTA
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UFR
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MNHN
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PNDB
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de.NBI
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SU

References