Image analysis using Galaxy

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.


Before diving into this topic, we recommend you to have a look at:


Lesson Slides Hands-on Recordings Input dataset Workflows
Analyse HeLa fluorescence siRNA screen
End-to-End Tissue Microarray Image Analysis with Galaxy-ME
Introduction to image analysis using Galaxy
Nucleoli segmentation and feature extraction using CellProfiler
Object tracking using CellProfiler
FAIR Bioimage Metadata
REMBI - Recommended Metadata for Biological Images – metadata guidelines for bioimaging data

Galaxy instances

You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.

You can also use the following Docker image for these tutorials:

docker run -p 8080:80

NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.

It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password password to access everything.

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoThomas Wollmann avatar Thomas Wollmann

For any question related to this topic and the content, you can contact them or visit our Gitter channel.


This material was contributed to by:

orcid logoAllison Creason avatar Allison Creasonorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoKrzysztof Poterlowicz avatar Krzysztof PoterlowiczYi Sun avatar Yi Sunorcid logoKatarzyna Kamieniecka avatar Katarzyna Kamienieckaorcid logoThomas Wollmann avatar Thomas WollmannJean-Karim Hériché avatar Jean-Karim HérichéLaura Cooper avatar Laura CooperJulia Jakiela avatar Julia Jakielaorcid logoBeatriz Serrano-Solano avatar Beatriz Serrano-Solanoorcid logoCameron Watson avatar Cameron Watsonorcid logoLeonid Kostrykin avatar Leonid Kostrykin


These individuals or organisations provided funding support for the development of this resource