Proteomics: MaxQuant workflow

Run Workflow in Galaxy

• Galaxy Australia
• Galaxy Europe
• Galaxy Main
• Galaxy France
• Import to another server (≥23.0+ only!)

flowchart TD
  0["ℹ️ Input Dataset\nProtein_database"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nSample1"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Dataset\nSample2"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["MaxQuant"];
  0 -->|output| 3;
  1 -->|output| 3;
  2 -->|output| 3;
  4["Filter"];
  3 -->|proteinGroups| 4;
  5["Select"];
  4 -->|out_file1| 5;
  6["Cut"];
  5 -->|out_file1| 6;
  7["Sort"];
  6 -->|out_file1| 7;
  8["Compute"];
  6 -->|out_file1| 8;
  9["Sort"];
  7 -->|outfile| 9;
  10["Compute"];
  8 -->|out_file1| 10;
  11["Cut"];
  10 -->|out_file1| 11;
  12["Compute"];
  10 -->|out_file1| 12;
  13["Histogram"];
  12 -->|out_file1| 13;

Inputs

Outputs

Tools

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Hands-on: Importing a workflow

• Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
• Click on galaxy-upload Import at the top-right of the screen
• Provide your workflow
  • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
  • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
• Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Version History

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/maxquant-label-free/workflows/maxquant_label_free.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows