Main Metatranscriptomics Analysis

Run Workflow in Galaxy

• Galaxy Main
• Galaxy Australia
• Galaxy France
• Galaxy Europe
• Import to another server (≥23.0+ only!)

flowchart TD
  0["ℹ️ Input Dataset\nT1A_forward"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nT1A_reverse"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["🛠️ Subworkflow\nWorkflow 1: Preprocessing"];
  style 2 fill:#edd,stroke:#900,stroke-width:4px;
  0 -->|output| 2;
  1 -->|output| 2;
  3["🛠️ Subworkflow\nWorkflow 2: Community Profile"];
  style 3 fill:#edd,stroke:#900,stroke-width:4px;
  2 -->|Cutadapt on input datasets: Read 1 Output| 3;
  2 -->|Cutadapt on input datasets: Read 2 Output| 3;
  4["🛠️ Subworkflow\nWorkflow 3: Functional Information"];
  style 4 fill:#edd,stroke:#900,stroke-width:4px;
  2 -->|FASTQ interlacer pairs from input datasets| 4;
  3 -->|MetaPhlAn on input datasets: Predicted taxon relative abundances| 4;

Inputs

Outputs

Tools

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Hands-on: Importing a workflow

• Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
• Click on galaxy-upload Import at the top-right of the screen
• Provide your workflow
  • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
  • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
• Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Version History

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/metatranscriptomics/workflows/main_workflow.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows