Workflows
These workflows are associated with VGP assembly pipeline
To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.
VGP assembly: training workflow
Last updated Mar 29, 2022
Launch in Tutorial Mode
License:
None Specified, defaults to
CC-BY-4.0
Tests: ❌
Results: Not yet automated
flowchart TD 0["Input dataset"]; 1["Input dataset"]; 2["Input dataset"]; 3["Input dataset collection"]; 4["GFA to FASTA"]; 5["GFA to FASTA"]; 6["Cutadapt"]; 7["Busco"]; 8["Busco"]; 9["Purge overlaps"]; 10["Collapse Collection"]; 11["Meryl"]; 12["Map with minimap2"]; 13["Quast"]; 14["Map with minimap2"]; 15["Meryl"]; 16["Purge overlaps"]; 17["Meryl"]; 18["Merqury"]; 19["Purge overlaps"]; 20["GenomeScope"]; 21["Purge overlaps"]; 22["Replace"]; 23["Compute"]; 24["Bionano Hybrid Scaffold"]; 25["Concatenate datasets"]; 26["Busco"]; 27["Replace"]; 28["Compute"]; 29["Concatenate datasets"]; 30["Purge overlaps"]; 31["Map with minimap2"]; 32["Search in textfiles"]; 33["Advanced Cut"]; 34["Advanced Cut"]; 35["BWA-MEM2"]; 36["BWA-MEM2"]; 37["Replace"]; 38["Quast"]; 39["Busco"]; 40["Map with minimap2"]; 41["Purge overlaps"]; 42["Convert"]; 43["Filter and merge"]; 44["Purge overlaps"]; 45["Advanced Cut"]; 46["bedtools BAM to BED"]; 47["PretextMap"]; 48["Purge overlaps"]; 49["Sort"]; 50["Pretext Snapshot"]; 51["Busco"]; 52["Quast"]; 53["SALSA"]; 54["BWA-MEM2"]; 55["BWA-MEM2"]; 56["Busco"]; 57["Quast"]; 58["Filter and merge"]; 59["PretextMap"]; 60["Pretext Snapshot"]; 3 -->|output| 6; 4 -->|out_fa| 7; 5 -->|out_fa| 8; 5 -->|out_fa| 9; 6 -->|out1| 10; 6 -->|out1| 11; 6 -->|out1| 12; 5 -->|out_fa| 12; 5 -->|out_fa| 13; 4 -->|out_fa| 13; 6 -->|out1| 13; 9 -->|split_fasta| 14; 9 -->|split_fasta| 14; 11 -->|read_db| 15; 12 -->|alignment_output| 16; 15 -->|read_db| 17; 5 -->|out_fa| 18; 4 -->|out_fa| 18; 15 -->|read_db| 18; 16 -->|pbcstat_cov| 19; 16 -->|calcuts_cutoff| 19; 14 -->|alignment_output| 19; 17 -->|read_db_hist| 20; 19 -->|purge_dups_bed| 21; 5 -->|out_fa| 21; 20 -->|summary| 22; 20 -->|model_params| 23; 0 -->|output| 24; 21 -->|get_seqs_purged| 24; 21 -->|get_seqs_hap| 25; 4 -->|out_fa| 25; 21 -->|get_seqs_purged| 26; 22 -->|outfile| 27; 23 -->|out_file1| 28; 24 -->|ngs_contigs_scaffold_trimmed| 29; 24 -->|ngs_contigs_not_scaffolded_trimmed| 29; 25 -->|out_file1| 30; 10 -->|output| 31; 25 -->|out_file1| 31; 27 -->|outfile| 32; 28 -->|out_file1| 33; 28 -->|out_file1| 34; 1 -->|output| 35; 29 -->|out_file1| 35; 2 -->|output| 36; 29 -->|out_file1| 36; 29 -->|out_file1| 37; 29 -->|out_file1| 38; 10 -->|output| 38; 29 -->|out_file1| 39; 30 -->|split_fasta| 40; 30 -->|split_fasta| 40; 31 -->|alignment_output| 41; 32 -->|output| 42; 36 -->|bam_output| 43; 35 -->|bam_output| 43; 41 -->|pbcstat_cov| 44; 41 -->|calcuts_cutoff| 44; 40 -->|alignment_output| 44; 42 -->|out_file1| 45; 43 -->|outfile| 46; 43 -->|outfile| 47; 44 -->|purge_dups_bed| 48; 25 -->|out_file1| 48; 46 -->|output| 49; 47 -->|pretext_map_out| 50; 48 -->|get_seqs_purged| 51; 21 -->|get_seqs_purged| 52; 48 -->|get_seqs_purged| 52; 10 -->|output| 52; 49 -->|out_file1| 53; 37 -->|outfile| 53; 1 -->|output| 54; 53 -->|scaffolds_fasta| 54; 2 -->|output| 55; 53 -->|scaffolds_fasta| 55; 53 -->|scaffolds_fasta| 56; 53 -->|scaffolds_fasta| 57; 10 -->|output| 57; 55 -->|bam_output| 58; 54 -->|bam_output| 58; 58 -->|outfile| 59; 59 -->|pretext_map_out| 60;
Importing into Galaxy
Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!Hands-on: Importing a workflow
- Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
- Click on galaxy-upload Import at the top-right of the screen
- Provide your workflow
- Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
- Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
- Click the Import workflow button
Below is a short video demonstrating how to import a workflow from GitHub using this procedure: