Assembly

DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a ‘Reference Sequence.’ We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.
Lesson Slides Hands-on Recordings Input dataset Workflows
An Introduction to Genome Assembly
An introduction to get started in genome assembly and annotation
Assembly of metagenomic sequencing data
assembly metagenomics microgalaxy
Assembly of the mitochondrial genome from PacBio HiFi reads
biodiversity
Chloroplast genome assembly
plants nanopore jbrowse1 biodiversity
De Bruijn Graph Assembly
Decontamination of a genome assembly
biodiversity
Deeper look into Genome Assembly algorithms
ERGA post-assembly QC
plants animals genome assembly QC biodiversity
Genome Assembly Quality Control
assembly quality control biodiversity
Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data)
nanopore assembly amr microgalaxy
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
illumina assembly microgalaxy
Genome assembly and assembly QC - Introduction short version
Genome assembly using PacBio data
assembly pacbio biodiversity
Hybrid genome assembly - Nanopore and Illumina
assembly nanopore illumina denovo genomics
Large genome assembly and polishing
assembly polishing nanopore plants biodiversity
Making sense of a newly assembled genome
gmod prokaryote microgalaxy jbrowse1
Unicycler Assembly
prokaryote microgalaxy biodiversity
Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads
covid19 biodiversity
Using the VGP workflows to assemble a vertebrate genome with HiFi and Hi-C data
pacbio eukaryote VGP biodiversity
Vertebrate genome assembly using HiFi, Bionano and Hi-C data - Step by Step
pacbio eukaryote VGP biodiversity

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Editorial Board

This material is reviewed by our Editorial Board:

Anton Nekrutenko avatar Anton Nekrutenkoorcid logoDelphine Lariviere avatar Delphine Lariviere

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Contributors

This material was contributed to by:

orcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAnna Syme avatar Anna SymeGiulio Formenti avatar Giulio Formentiorcid logoWolfgang Maier avatar Wolfgang MaierGrace Hall avatar Grace Hallorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoNate Coraor avatar Nate Coraororcid logoStéphanie Robin avatar Stéphanie Robinorcid logoLaura Leroi avatar Laura Leroiorcid logoTom Brown avatar Tom Brownorcid logoDelphine Lariviere avatar Delphine LariviereAnton Nekrutenko avatar Anton NekrutenkoBazante Sanders avatar Bazante Sandersorcid logoHelena Rasche avatar Helena Rascheorcid logoSaskia Hiltemann avatar Saskia HiltemannMatúš Kalaš avatar Matúš KalašMarcella Sozzoni avatar Marcella Sozzoniorcid logoSimon Gladman avatar Simon Gladmanorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoErwan Corre avatar Erwan Correorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoPolina Polunina avatar Polina Poluninaorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoTeresa Müller avatar Teresa Müllerorcid logoMiaomiao Zhou avatar Miaomiao ZhouBrandon Pickett avatar Brandon Pickettorcid logoSolenne Correard avatar Solenne CorreardVerena Moosmann avatar Verena MoosmannFabian Recktenwald avatar Fabian Recktenwaldorcid logoAlex Ostrovsky avatar Alex Ostrovskyorcid logoBjörn Grüning avatar Björn Grüningorcid logoBérénice Batut avatar Bérénice Batutorcid logoLinelle Abueg avatar Linelle Abueg

Funding

These individuals or organisations provided funding support for the development of this resource

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The University of Melbourne
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Avans
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Australian BioCommons
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Melbourne Bioinformatics
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ABRomics
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Gallantries
This project (2020-1-NL01-KA203-064717) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.