Visualization Of RNA-Seq Results With Volcano Plot
transcriptomics-rna-seq-viz-with-volcanoplot/rna-seq-viz-with-volcanoplot
Launch in Tutorial Mode
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flowchart TD 0["ℹ️ Input Dataset\nDE results"]; style 0 stroke:#2c3143,stroke-width:4px; 1["ℹ️ Input Dataset\nVolcano genes"]; style 1 stroke:#2c3143,stroke-width:4px; 2["Volcano plot highlighting significant"]; 0 -->|output| 2; 3["Volcano plot labelling top 10"]; 0 -->|output| 3; 4["Volcano plot labelling genes of interest"]; 0 -->|output| 4; 1 -->|output| 4; 16aef53e-11fd-4def-a7fb-6cf9a98f0902["Output\nvolcano_pdf"]; 4 --> 16aef53e-11fd-4def-a7fb-6cf9a98f0902; style 16aef53e-11fd-4def-a7fb-6cf9a98f0902 stroke:#2c3143,stroke-width:4px;
Inputs
Input | Label |
---|---|
Input dataset | DE results |
Input dataset | Volcano genes |
Outputs
From | Output | Label |
---|---|---|
toolshed.g2.bx.psu.edu/repos/iuc/volcanoplot/volcanoplot/0.0.5 | Volcano Plot | Volcano plot labelling genes of interest |
Tools
Tool | Links |
---|---|
toolshed.g2.bx.psu.edu/repos/iuc/volcanoplot/volcanoplot/0.0.5 | View in ToolShed |
To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.
Importing into Galaxy
Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!Hands-on: Importing a workflow
- Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
- Click on galaxy-upload Import at the top-right of the screen
- Provide your workflow
- Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
- Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
- Click the Import workflow button
Below is a short video demonstrating how to import a workflow from GitHub using this procedure:
Video: Importing a workflow from URL
Version History
Version | Commit | Time | Comments |
---|---|---|---|
8 | 470fa5796 | 2021-06-18 20:12:15 | Fix name |
7 | 086ac3f50 | 2021-06-18 20:09:12 | Update workflow |
6 | 2e2cb42d8 | 2020-10-04 11:59:11 | add tests from workflow-testing for blockclust and rna-seq-viz-with-volcano-plot, edit workflows |
5 | 667ff3de9 | 2020-01-22 10:59:29 | annotation |
4 | f7f017f33 | 2020-01-15 12:40:00 | dna and rna capitals |
3 | eb4d724e0 | 2020-01-15 10:41:35 | Workflow renaming |
2 | faf6d298a | 2019-12-12 13:02:33 | unflatten workflows |
1 | 47566efc6 | 2019-07-05 14:38:11 | Add workflow to volcanoplot tutorial |
For Admins
Installing the workflow tools
wget https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/rna-seq-viz-with-volcanoplot/workflows/rna-seq-viz-with-volcanoplot.ga -O workflow.ga workflow-to-tools -w workflow.ga -o tools.yaml shed-tools install -g GALAXY -a API_KEY -t tools.yaml workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows