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--- # Introduction to Metabolomics --- ## Requirements Before diving into this slide deck, we recommend you to have a look at: - (/training-material/topics) --- # Metabolomics .pull-left[!(../images/slide-intro-wheel.png)] .pull-right[!(../images/slide-intro-omics.png)] « Quantitative measurement of the dynamic multiparametric metabolic response of living systems to pathophysiological stimuli or genetic modification » (Nicholson, 1999) --- ## Definitions - Metabolomics - Newly emerging field of 'omics' research - Comprehensive and simultaneous systematic determination of metabolite levels in the metabolome and their changes over time as a consequence of stimuli - Metabolome - Refers to the complete set of small-molecule metabolites - Dynamic - Metabolites - Intermediates and products of metabolism - Examples include antibiotics, pigments, carbohydrates, fatty acids and amino acids - Primary and secondary metabolites --- ## Definitions .pull-left[ Metabolo **_l_** omics .left[ Metabolic fingerprints based on comprehensive quantification = Comprehensive and simultaneous systematic determination of metabolite levels in a biological system (cell, tissue, fluid) ]] .pull-right[ Metabolo **_n_** omics .left[ Description of metabolic changes = Detection of disrupted metabolic pathways ]] --- ## Metabolomics : the world of small molecules .pull-left[ **Physico-chemical diversity** - Comparison with - ADN/ARN: 4 bases - Proteins: 20 amino-acids - Common physico-chemical propreties - Extraction/Analysis: easy automation - Metabolites - Number ≥ 150 000 in Nature - Most of them not identified - Wide diversity of structures, functional groups, physico-chemical properties - ex: lipids, sugars, aminoacides, etc.) - High turn-over rates (<sec) - Range from 1 pM to 100 mM ] .pull-right[ **Molecular mass range** !(../images/slide-intro-molecular_mass_range.png) <small>80% of metabolites with mol. mass ≤ 600 (E. coli, S. cerevisiae)</small> ] --- ## Analytical challenge !(../images/slide-intro-metabolome_pathway.png) --- ## Complementary analytical tools ### For metabolome investigation !(../images/slide-intro-ms_nmr_complementarity.png) --- ## Strategy: untargeted metabolomics or metabolic fingerprinting !(../images/slide-intro-untargeted_metabolomics.png) --- ## Strategy: targeted metabolomics Qualitative and quantitative analysis !(../images/slide-intro-targeted_metabolomics.png) --- ## Related tutorials --- ## Thank you! This material is the result of a collaborative work. Thanks the [Galaxy Training Network](https://wiki.galaxyproject.org/Teach/GTN) and all the contributors (Gildas Le Corguillé, Cécile Canlet) !