Assembly-Hifi-Trio-phasing-VGP5
- Author(s):
- Galaxy
- VGP
- Release: 0.2
- License: CC-BY-4.0
- UniqueID: 3cacf798-0f32-43b9-82c0-2d8f76312c80
Assembly with Hifi reads and Trio Data
Generate phased assembly based on PacBio Hifi Reads using parental Illumina data for phasing
Inputs
- Hifi long reads [fastq]
- Concatenated Illumina reads : Paternal [fastq]
- Concatenated Illumina reads : Maternal [fastq]
- K-mer database [meryldb]
- Paternal hapmer database [meryldb]
- Maternal hapmer database [meryldb]
- Genome profile summary generated by Genomescope [txt]
- Genome model parameters generated by Genomescope [tabular]
- Homozygous read coverage (Estimated from the Genomescope model if not provided)
- Lineage of the species being assembled
- Bloom Filter
- Name of first haplotype
- Name of second haplotype
Outputs
- Haplotype 1 assembly
- Haplotype 2 assembly
- QC: BUSCO report for both assemblies
- Merqury report for both assemblies
- Assembly statistics for both assemblies
- Nx Plot for both assemblies
- Size plot for both assemblies
Changelog
[0.2] 2024-08-12
Added
- Workflow report
Changed
- Expose the Busco lineage database parameter
- Fix a bug happening when merging assembly statistics : The presence of the '#' character caused the tables to join incorrectly, and the numbers did not match the metrics
[0.1.8] 2024-07-22
Automatic update
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.9+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.9+galaxy1
[0.1.7] 2024-07-15
Automatic update
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.8+galaxy1
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.9+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.9+galaxy0
[0.1.6] 2024-05-20
Automatic update
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.8+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.8+galaxy1
[0.1.5] 2024-05-06
Automatic update
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy1
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy1
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy1
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/9.3+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/9.3+galaxy1
[0.1.4] 2024-04-22
Added
- Add Homozygous Read coverage parameter and calculation
- Expose Lineage parameter
Automatic update
toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3
was updated totoolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy4
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.8+galaxy0
[0.1.3] 2024-03-25
Manual update
- Add gff output to Busco
- Add histogram output to Merqury
- Label subworkflows
Automatic update
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/9.3+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1
[0.1.2] 2023-11-14
Automatic update
toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1
was updated totoolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0
toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.16.1+galaxy4
was updated totoolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0
toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0
toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2
was updated totoolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3
toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2
was updated totoolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0
toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0
was updated totoolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1
toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0
was updated totoolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1
[0.1.1] 2023-11-20
- Fix author in dockstore
[0.1] - 2023-09-27
Creation of workflow for Trio assembly.
The following tools are required to run this workflow.
This will eventually be a pretty page with links to each tool in the (new) toolshed, etc.
- toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.9+galaxy1
- toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/2.0
- toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1
- toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1
- Cut1
- toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy1
- param_value_from_file
- Convert characters1
- toolshed.g2.bx.psu.edu/repos/iuc/pick_value/pick_value/0.2.0
- toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.9+galaxy0
- toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
- toolshed.g2.bx.psu.edu/repos/iuc/bandage/bandage_image/2022.09+galaxy4
- toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0
- toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy4
- toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy1
- __EXTRACT_DATASET__
- join1
- toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cut_tool/9.3+galaxy1
- toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy1